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Tris base

is a Star Product of
Horay Industry Co., Ltd.

Horay Industry Co., Ltd.


General information
Registration name: Tris base
Other product names:
Tris base solution
Trometamol
Tris, Reag
THAM
Tromethamine
tris-(Hydroxymethyl)aminomethane
2-Amino-2-(hydroxymethyl)propane-1,3-diol
tris-(Hydroxymethyl)aminomethane, Reag.
Trisamine
2-Amino-2-(hydroxymethyl)-1,3-propanediol
TRIS
Tris, ultra pure
TRIS buffer
 
CAS number 77-86-1 Structure
EINECS: 201-064-4
Formula: C4H11NO3

Detailed information on the Star Product
MF: C4H11NO3
MW: 121.14

Appearance:White crystal powder,Solubility Colorless and clarification
Content:≥99.5%
Melting point:168.0~172.0℃
Loss on drying:≤0.2%
pH:10.0~11.5
Fe3+ :≤1ppm
SO42-:≤10ppm
Cl-:≤3ppm
Nickel salt:≤10ppm
Heavy metal:≤5ppm
Ultraviolet absorbance/280 nm(40% aq.):≤0.07
Ultraviolet absorbance/290 nm(40% aq.):≤0.20
Ultraviolet absorbance/400 nm(40% aq.):≤0.020

Tris base is widely used in acute metabolic and respiratory acidemia. It is an alkaline buffer that has a good buffering effect on metabolic acidosis and enzyme activity reactions. Tris is commonly used as a biological buffer, often formulated with pH values of 6.8, 7.4, 8.0, 8.8. Its structural formula and pH value vary greatly with temperature. Generally speaking, for every one degree increase in temperature, the pH value decreases by 0.03. Tris is widely used in the preparation of buffer solutions in biochemical and molecular biology experiments. For example, the commonly used TAE and TBE buffer solutions (used for nucleic acid dissolution) in biochemical experiments require the use of Tris; Due to its amino group content, it can undergo condensation reaction with aldehydes. The pH of Tris alkali aqueous solution is around 10.5. Generally, hydrochloric acid is added to adjust the pH value to the desired value to obtain a buffer solution with that pH value. However, attention should also be paid to the effect of temperature on the pKa of Tris. Due to Tris buffer being a weakly alkaline solution, DNA is deprotonated in such a solution, thereby improving its solubility. People often add EDTA to Tris hydrochloric acid buffer to make "TE buffer", which is used for DNA stabilization and storage. If the acid solution used to adjust the pH value is replaced with acetic acid, a "TAE buffer" (Tris/Acetate/EDTA) is obtained, while replacing it with boric acid results in a "TBE buffer" (Tris/Borate/EDTA). These two types of buffers are commonly used in nucleic acid electrophoresis experiments. TAE and TBE, which are composed of Tris, are the most commonly used reagents for DNA electrophoresis. TE (pH 8.0) is mainly used to dissolve DNA (TE is a combination of Tris and EDTA). Tris buffer is not only widely used as a solvent for nucleic acids and proteins, but also has many important applications; It is used for protein crystal growth under different pH conditions. The low ion strength of Tris buffer can be used for the formation of intermediate fibers in the lamin of C. elegans, and Tris is also one of the main components of protein electrophoresis buffer. In addition, Tris is also an intermediate in the preparation of surfactants, vulcanization accelerators, and some drugs, and it is often used as a titration standard.
*Tris is mainly used as a pharmaceutical intermediate; Can be used as a biological buffer and a reference substance for acidometric titration; Also used as an intermediate in biochemical reagents and phosphomycin; It can also be used as a vulcanization promoter, cosmetic (cream, detergent), mineral oil, paraffin emulsifier, and biological buffer.

Information on the supplier

Horay Industry Co., Ltd.

Horay Industry Co., Ltd. founded in 2013,which is located in the beautiful coastal city Qingdao. It is a technology-based production enterprise integrating R&D, production and sales of fine chemical additives, surfactants, solvents and catalysts,etc. Our company strictly controls from raw material procurement to product delivery to ensure that product quality meets relevant standards. Horay also actively establishes cooperation with higher education institutions and research institutes,with strong technical support,complete production facilities,and a high-quality production management team, casting a demonstration enterprise for the new generation of chemical additives industry.

By leveraging the geographical advantages of Qingdao Port, our various series of products are exported to Asia, Europe, and the Americas, we established a mature procurement system and marketing network for our customers. We adheres to the corporate philosophy of “Excellent in quality Sincere in heart”, and we are dedicated to service customers with excellent solutions. We sincerely look forward to working with our customers to create brilliance together! We warmly welcome guests and friends from all walks of life at home and abroad to visit and patronize us, seek common development, and grow and progress together,grow and progress together!
AddressHoray Industry Co., Ltd.
No. 31 Longcheng Road, Shibei District
Qingdao City, Shandong Province,
Phone +86-13355429055
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